A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Abstract a methodology for the in vitro and in vivo establishment of aniba perutilis, a native colombian tree which is cur. Ms nutrient media containing iba at all tested concentrations table 1. Pdf caracterizacion fisiologica del enraizamiento in. Efficient method of micropropagation and in vitro rooting of. In vitro hyperhydric plantlets presented numerous anatomical. The rooting experiments were conducted in vitro and ex vitro conditions with micropropagated shoots approximately 12 cm long obtained from the fifth subcultures. The ms basal medium containing half strength of macronutrients supplemented with 3% wv sucrose and 0. In vitro shoot proliferation and in vitro and ex vitro.
In vitro normal plantlets displayed good development and survived during the acclimatization stage. Nydia del rivero bautista 1,2, daniel agramonte penalver 1, raul barbon rodriguez, wilder camacho chiu 2 y. Initiation of roots emerged 14 days after planting. Qualitative and quantitative changes in putrescine, spermidine, and spermine tissue contents, during the rooting process and their correlation with other biochemical markers and morphological changes, provided the rationale. Qualitative and quantitative changes in putrescine, spermidine, and spermine tissue contents, during the rooting process and their correlation with other biochemical markers and morphological changes, provided the rationale for their use during the study of a. Comino crespo, establecimiento in vitro, in vivo, miniesquejes, recalcitrancia. In tissue culture, the propagated shoots of teak may be rooted ex vitrum like conventional cuttings or in.
In vitro clonal propagation and cutting rooting of native cotton gossypium barbadense l. Rooting and acclimatization of apuleia leiocarpa plantlets. Cultivo in vitro del aguacatero persea americana mill. In vitro shoot proliferation and in vitro and ex vitro root. Establishment and in vitro clonal propagation of the. The existence of welldeveloped and healthy root system of in vitro plants was a very important step for subsequent acclimatization in conditions ex vitro. Efficient method of micropropagation and in vitro rooting. Vitis, apices, micropropagacion, segmentos nodales, explantes axilares, plantulas crecidas in vitro. Michael kane environmental horticulture department university of florida rapid clonal in vitro propagation of plants from cells, tissues or organs cultured aseptically on defined media contained in culture vessels maintained under controlled conditions of light and temperature micropropagation in vitro propagation. Anatomy of normal and hyperhydric leaves and shoots of in. During the rooting testing process, the culture medium used was the one formulated by. In vitro rooting could be induced in the rejuvenation and rooting medium, which was 12 om supplemented with 2. This study was carried out to assess in vitro plant growth and rooting of dendrobium nobile seedlings in culture media containing different sucrose concentrations. The anatomy of normal and hyperhydric in vitro shoots and leaves from micropropagated simmondsia chinensis link.
Dendrobium nobile plantlets derived from seeds germinated in vitro were used with a mean initial height of 1. Original paper effect of silver nitrate on in vitro root. Physiological and developmental problems encountered by in vitro cultured plants michael w. An in vitro rooting protocol for producing nothofagus nervosa microplants using successive steps during tissue culture is reported. Consuelo rojasidrogo1, carola cuzquencabrejos1, guillermo e. Establishment and in vitro clonal propagation of the spanish. It was hard to survive for seedlings of in vitro rooting after transplanting. Physiological and developmental problems encountered by in. In vitro dendrobium nobile plant growth and rooting in. None of the published protocols fully satis es the requirementsfor commercialapplicationin spite ofthe progress made on tissue culture of teak since the 1970s8,18. Skoog, woody plant medium, mascarenhas, enraizamiento, hormonas.